@article {3215668,
	title = {Surface-Controlled Sialoside-Based Biosensing of Viral and Bacterial Neuraminidases},
	journal = {Langmuir},
	year = {2024},
	abstract = {Neuraminidases (NA) are sialic acid-cleaving enzymes that are used by both bacteria and viruses. These enzymes have sialoside structure-related binding and cleaving preferences. Differentiating between these enzymes requires using a large array of hard-to-access sialosides. In this work, we used electrochemical impedimetric biosensing to differentiate among several pathogene-related NAs. We used a limited set of sialosides and tailored the surface properties. Various sialosides were grafted on two different surfaces with unique properties. Electrografting on glassy carbon electrodes provided low-density sialoside-functionalized surfaces with a hydrophobic submonolayer. A two-step assembly on gold electrodes provided a denser sialoside layer on a negatively charged submonolayer. The synthesis of each sialoside required dozens of laborious steps. Utilizing the unique protein{\textendash}electrode interaction modes resulted in richer biodata without increasing the synthetic load. These principles allowed for profiling NAs and determining the efficacy of various antiviral inhibitors.},
	author = {I, Alshanski and T, Toraskar and D, Gordon-Levitan and M, Massetti and P, Jain and M, Vaccaro and R, Kikkeri and M, Hurevich and S, Yitzchaik}
}
